Tag Archive | CHAPTER 2

Chapter 2 – The Preoperative Evaluation

15 Chapter 2 – The Preoperative Evaluation James Blaugrund Dario Kunar Preoperative evaluation of surgical patients is, in its broadest sense, an extension of the diagnostic process. The surgeon should strive to determine the extent of disease; prove the necessity of surgery or clearly demonstrate its benefit to the patient; optimize the choice of surgical […]

CHAPTER 2 EXAMINATION OF THE BLOOD

CHAPTER 2 EXAMINATION OF THE BLOOD Williams Hematology CHAPTER 2 EXAMINATION OF THE BLOOD DANIEL H. RYAN Quantitative Measures of Hematopoietic Elements in the Blood Red Cells Leukocytes Platelets Morphologic Examination of the Blood Red Cell Morphology Platelet Morphology Leukocyte Morphology Leukocyte Inclusions Leukocyte Artifacts The Need for Examination of the Blood Film Chapter References […]

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE Principles and Practice of Endocrinology and Metabolism CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE MEHBOOB A. HUSSAIN AND JOEL F. HABENER Cloning of Genes Genomic Libraries and Gene Isolation Gene Amplification by Polymerase Chain Reaction Variations of Polymerase Chain Reaction Approaches to the Quantitative Assessment of Gene Expression […]

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE(cont’4)

Retroviral Vectors. The Moloney murine leukemia virus (MoMuLV) was the first virus used in developing a vector system for gene transfer. Parts of the retroviral genome that are involved in the replication of the MoMuLV—gag, pol, and env—are removed and replaced by a gene of interest. What remains of the retrovirus are the long terminal […]

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE(cont’3)

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE (cont’2)

PROTEIN-PROTEIN INTERACTION ASSAYS A number of different assays are used to determine and evaluate protein-protein interactions. Two in vitro assays are coimmunoprecipitation and polyhistidine-tagged glutathione sulfonyl transferase (GST) pull-down. Two in vivo assays are the yeast and mammalian two-site interaction assays. Coimmunoprecipitation. The commonly used coimmunoprecipitation assay makes use of antisera to specific proteins. In […]

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE

CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE Principles and Practice of Endocrinology and Metabolism CHAPTER 2 MOLECULAR BIOLOGY: PRESENT AND FUTURE MEHBOOB A. HUSSAIN AND JOEL F. HABENER Cloning of Genes Genomic Libraries and Gene Isolation Gene Amplification by Polymerase Chain Reaction   Variations of Polymerase Chain Reaction Approaches to the Quantitative Assessment of Gene […]